Environmental Genomics, inc
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    • VISION AND MISSION
    • ONE HEALTH EPIGENOMICS
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  • ONE HEALTH Epigenomics and Microbiomes, From Soil to People
    • ONE HEALTH PROJECTS >
      • THE MANGROVE EPIGENOME (MangroveENCODE) PROJECT >
        • mangrove conservation
        • mangrove genomes
        • transposable elements of mangroves
        • contaminants of mangroves (metals, glyphosate, Bt, EDCs)
      • THE SHRIMP EPIGENOME (ShrimpENCODE) PROJECT >
        • penaeid shrimp genomes
        • pilot genome sequence of first SPF Penaeus vannamei
        • epigenetic genes of shrimp
        • transposable elements of Penaeus vannamei
        • contaminants of wild and farmed shrimp (metals, glyphosate, Bt, EDCs)
        • glyphosate-free shrimp feeds
        • transgenerational toxicological effects of glyphosate on shrimp
      • THE COASTAL PEOPLE EPIGENOME (ChildrenENCODE) PROJECT >
        • Shrimp Scampi
        • glyphosate health effects >
          • glyphosate and congenital malformations
          • glyphosate and non-alcoholic fatty liver disease
          • glyphosate and non-Hodgkins lymphoma
  • ONE HEALTH NEWS!
  • PUBLICATIONS
  • MEETINGS - ADWARDS
  • CONTACT US

Why ​ONE HEALTH?

In general, ‘One Health’ is defined as a collaborative, multisectoral, and trans-disciplinary approach - working at local, national, and global levels – to achieve optimal health and well-being outcomes recognizing the interconnections between people, animals, plants, and their shared environment (One Health Commision). 
The One Health concept originated in medical and veterinary spheres as an open call for collaboration between these disciplines or professions and those of environmental and social science (Fig.1), but the inclusion of environmental science has proven weak so far (Destoumieux-Garzón et al. 2018) and suggestions were made to address inter- and trans-disciplinary solutions aimed at improving management of health risks and benefits associated with nature (Antoine-Moussiaux et al. 2019). ​
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Fig. 1. The One Health concept: a holistic, transdisciplinary, and multisectoral approach of Health. Taken from Destoumieux-Garzón D, P Mavingui, G Boetsch, J Boissier, F Darriet, P Duboz, C Fritsch, P Giraudoux, F Le Roux, S Morand, C Paillard, D Pontier, C Sueur, Y Voituron. 2018. The One Health Concept: 10 Years Old and a Long Road Ahead. Front Vet Sci. 2018; 5: 14.
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Fig 2. The ONE HEALTH Program of the FUCOBI Foundation of Ecuador. Taken from Acacia Alcivar-Warren, Kathy F.J. Tang and Miriam Alcivar-Arteaga. 2020. Shellfisheries Association Quarterly Newsletter - Summer issue. Article addresses the holistic concept of ONE HEALTH, epigenetics, shellfish and coronavirus epigenetics, transposable elements (TEs), coronaviruses in wastewater, and case studies about endogenous shrimp viruses (WSSV, IHHNV, TSV) and horizontal transfer and epidemiology of Vibrios in shellfish, and the need for long-term risk assessments.
EGI is helping to address environmental, animal and human health issues through the program ‘UNA SALUD / ONE HEALTH Epigenomics and Microbiomes: Somos lo que comemos / We are what we eat’ of the Foundation for Conservation of Biodiversity (FUCOBI) of Ecuador (Fig. 2). Our long-term goal is to conserve biodiversity of healthy terrestrial and aquatic ecosystems (agricultural soil, rivers, mangroves, wetlands), to maintain healthy animals (shrimp/shellfish, fish), to protect people’s health. Collaborators include local citizens and international experts on mangrove conservation and climate change, shrimp genomics and epigenomics, shellfish biologists and ecologists, clinicians and veterinarians with expertise in zoonotic diseases, epidemiology and public health, toxicologists and water sanitation experts, among others. Our immediate goal is to help educate the next generation of scientists and clinicians on the need to apply One Health and (epi)genetic epidemiology tools in environmental and public health risk assessments.
Basic principles about (epi)genetic epidemiology, transposable elements and transgenerational epigenetic inheritance (TGEI) are summarized below, with examples of epigenetic modifications of infection with Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-Cov-2), transposable elements of vertebrates, and proposed TGEI studies for shrimp, respectively.

GENETIC EPIDEMIOLOGY

The relatively young field of genetic epidemiology brings together genetics, epidemiology and biostatistics to identify and implement the best study designs and statistical analyses for identifying genes controlling risk to complex and heterogeneous diseases. The field has moved quickly over the past 40 years partly because the technology of genotyping and sequencing has forced it to adapt while adhering to the fundamental principles of genetics and epigenetics. The available tools for genetic epidemiology have expanded from a genetic focus (i.e. considering one gene at a time) to a genomic focus (i.e. considering the entire genome), and now they must further expand to integrate information from other "-omics" (e.g. epigenomics, transcriptomics) both at the individual level and at the population level. The gene and environmental interactions across populations can now be evaluated to better understand exposure and the heterogeneity in disease risk.
 

ONE HEALTH EPIDEMIOLOGY:
​SARS-Cov-2 IN WASTEWATER

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Fig. 3. The emergence of SARS-CoV-2 and the outbreak of COVID-19. The figure depicts a hypothesized origin of the virus and a generalised route of transmission of the epidemic zoonotic coronavirus. Taken from El Zowalaty and Järhult. 2020. From SARS to COVID-19: A previously unknown SARS- related coronavirus (SARS-CoV-2) of pandemic potential infecting humans – Call for a One Health approach. One Health. 2020 Jun; 9: 100124Published online 2020 Feb 24. doi: 10.1016/j.onehlt.2020.100124 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7228230/

​Urgent actions are needed to conserve marine biodiversity because of contamination of terrestrial and marine ecosystems with disinfectants used to control coronavirus disease-2019 (COVID-19) pandemic. Since the emergence of SARS-Cov-2, an enveloped single-stranded RNA virus which causes COVID-19, EGI began helping the FUCOBI Foundation of Ecuador with the project ‘UNA SALUD / ONE HEALTH Epidemiology: Coronavirus in wastewater of selected towns of coastal provinces and the Galapagos Islands of Ecuador’. Ecuador was the first most seriously affected Latin American country by the ​COVID-19 affects people with underlining health conditions such as diabetes, obesity, hypertension, among others. 

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Fig. 4. Association of global crude oil, atmospheric pollutants and pesticide production with population growth and chronic disease trends. From Tsatsakis et al. 2020. COVID-19, an Opportunity to Reevaluate the Correlation Between Long-Term Effects of Anthropogenic Pollutants on Viral epidemic/pandemic Events and Prevalence. Food Chem Toxicol 2020 Jul;141:111418. doi: 10.1016/j.fct.2020.111418. Epub 2020 May 11.
Long-term exposure to anthropogenic pollutants, among other risk factors, may have a positive association with chronic diseases such as neurodegenerative disorders (e.g., Parkinson's disease) and diabetes, breast cancer, non-Hodgkin's lymphoma, cancer, leukemia, obesity, congenital heart defects, hypertension,and increased vulnerability to microbial and viral infections and their mortality (Figs. 4 and 5).

​There is a need for anthropological and scientific approach for the evaluation of the association of the global production of crude oil, atmospheric pollutants, and pesticides with the population growth and chronic diseases trends.


EGI is concerned about the threat to aquatic ecosystems caused by disinfectants using during the COVID-19 pandemic (Zhang et al. 2020, https://science.sciencemag.org/content/368/6487/146), and we are offering research awards to veterinary medical students to address these issues.
​

EPIGENETICS

Epigenetics is the regulation of gene expression through alterations in DNA or associated factors (other than the DNA sequence). These factors control the diverse manifestations of diseases. Advances in epigenetics link genetics to the environment and disease (Fig.6).
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Fig. 6. Epigenetic components.- DNA methylation, histone methylation and acetylation, non-coding RNAs (microRNA)) are affected by various factors and processes: development (in uterus, childhood), environmental chemicals, drugs / pharmaceuticals, aging, diet, which impact health (cancer, autoimmune disease, mental disorders, diabetes, others).
Examples of epigenetic modifications by infections with highly pathogenic coronaviruses and influenza viruses are presented in Figs. 7 and 8. 

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Fig. 7. Epigenetic landscape during coronavirus infection. (A) Histone tails are targets for post-translational covalent modification. Particular lysine residues (K) can be methylated, acetylated, phosphorylated, and ubiquitinated, and particular serine residues (S) can be also phosphorylated. (B) DNA is being methylated by transferring a methyl group to the C-5 position on cytosine bases. (C) miRNA, usually 22nt long, bind the 3’-end of their target mRNA with their seed region and mediate the degradation of the mRNA by incorporation into RISC. DNMT: DNA methyltransferase; mRNA: messenger RNA; miRNA: microRNA; RISC: RNA-induced silencing complex; K: lysine; S: serine; T: threonine. Taken from Schäfer & Baric 2018.Epigenetic Landscape during Coronavirus Infection. Pathogens. 2017 Mar;6(1):8.
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Fig. 8. Epigenetic modification by infection with highly virulent respiratory viruses, pathogenic influenza viruses and coronavirus. Epigenetic modification induces changes in the basal state of host chromatin. Infection with H1N1-09 (blue) and SARS-CoV (red) results in enrichment of H3K4me3 incorporation (green ovals) and depletion of H3K27 (red diamonds), and therefore in open, transcription-active chromatin. In contrast, H5N1-VN1203 and Middle East respiratory syndrome coronavirus (MERS-CoV) infection drives H3K27me3 enrichment and depletes H3K4me3 for a subset of genes, favoring a closed chromatin conformation that inhibits interferon-stimulated gene (ISG) expression. Taken from Schäfer & Baric 2018.Epigenetic landscape during coronavirus infection. Pathogens. 2017 Mar;6(1):8.”
Epigenetic changes after infection with shrimp viruses (WSSV, IHHNV) and Vibrios that cause AHPND disease are presented in The Shrimp Epigenome (ShrimpENCODE) Project section.

TRANSPOSABLE ELEMENTS (TEs)

Transposable elements are divided in two classes: (A) Transposons, which transpose directly from DNA to DNA and (B) Retrotransposons, that use reverse transcriptase to transpose by means of an RNA intermediate  (Figs. 9 and 10).
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Fig. 9. Simplified version of transposed mobile elements. Left (a) insertion sequence or transposon that transpose via DNA intermediary. Right (b) Retrotransposon that transpose via RNA intermediate.
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Fig. 10. Examples of (a) DNA transposon (‘cut’ and ‘paste’ TE); (b) LTR retrotransposon (replicative retrotransposition), and (c) Non-LTR retrotransposon (target-site primed reverse transcription), showing the human LINE-1 (L1) non-LTR retrotransposon.

TRANSPOSABLE ELEMENTS OF SPECIFIC PATHOGEN-FREE (SPF)

 P. vannamei
Transposable elements play essential roles in epigenetics and regulation of the immune system of shrimp. Over 300 repetitive sequences / transposable elements (TEs) have been characterized from the first SPF P. vannamei produced in the USA by EGI collaborator, Dr. Weidong Bao, at the Genetics Information Research Institute (GIRI), CA USA. For example, a hemocyanin promoter gene contains most of the Penelope-5_LV non-LTR retrotransposon. A transcript containing L1-like sequences was identified in transcriptome of ‘SPF’ P. vannamei infected with WSSV farmed in Brazil (Santos et al. 2019), but is not present in the draft genome sequence assembled for farmed P. vannamei from China (Alcivar-Warren, 2020). Some shrimp TEs represent endogenous virus of shrimp like white spot syndrome virus (WSSV), WSSV-EVE, and IHHNV-EVE. A chimeric Taura syndrome virus (TSV) sequence has also been found in P. vannamei farmed in the Philippines. 

helping to train the next generation

EGI support local and international students – citizens science projects, basic research, and laboratory teaching projects. We donate laboratory supplies and small equipment to collaborators for conservation genetics and disease diagnostics projects.
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ONE HEALTH EPIGENOMICS – A GLOBAL EDUCATIONAL INITIATIVE (OHEEI)

The OHEEI was initiated by high school students from Massachusetts to assess the adverse health effects to the environment, wildlife, pets, seafood, and people, caused by the antimicrobial herbicide Glyphosate (RoundUp) and other endocrine disrupting chemicals (EDCs) like metals and Bisphenol A (BPA). 
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SHRIMP SCAMPI 

A citizen science project to (a) review the scientific literature about epigenetic mechanisms associated with EDCs [Glyphosate (G), metals, BPA] associated with antimicrobial resistance, diabetes, obesity, brain development, and neural tube defects (NTDs), and (b) examine the levels of glyphosate and metals in fresh and frozen shrimp sold at USA supermarkets.
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ONE HEALTH EPIGENOMICS AWARDS FOR VETERINARY MEDICINE STUDENTS FROM LATIN AMERICA, AFRICA AND SOUTH EAST ASIA

-To diagnose pathogenic WSSV, non-pathogenic WSSV-EVE and endogenous Nimavirus (Nimav-1LVa).
-To study glyphosate’s impact on your health: exposed before birth, generational impacts, cancer (non-Hodgkin lymphoma), endocrine disruptor, gut microbiome disruptor, non-alcoholic fatty liver disease.
-TGEI -generational toxicology research – shrimp fed diets using native versus GM-based soybeans.
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LABORATORY TRAINING

Teaching Ecuadorian students to isolate DNA for genetic diversity and disease diagnosis studies.

TRAVEL AWARDS FOR STUDENTS TO ATTEND THE ANNUAL MEETING OF THE NATIONAL SHELFISHERIES ASSOCIATION (NSA) 

To present results about the topics included in our ONE HEALTH Epigenomics projects.
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Photo used under Creative Commons from Arkansas Agricultural Experiment Station
  • HOME
    • ABOUT US
    • VISION AND MISSION
    • ONE HEALTH EPIGENOMICS
  • SERVICES
  • ONE HEALTH Epigenomics and Microbiomes, From Soil to People
    • ONE HEALTH PROJECTS >
      • THE MANGROVE EPIGENOME (MangroveENCODE) PROJECT >
        • mangrove conservation
        • mangrove genomes
        • transposable elements of mangroves
        • contaminants of mangroves (metals, glyphosate, Bt, EDCs)
      • THE SHRIMP EPIGENOME (ShrimpENCODE) PROJECT >
        • penaeid shrimp genomes
        • pilot genome sequence of first SPF Penaeus vannamei
        • epigenetic genes of shrimp
        • transposable elements of Penaeus vannamei
        • contaminants of wild and farmed shrimp (metals, glyphosate, Bt, EDCs)
        • glyphosate-free shrimp feeds
        • transgenerational toxicological effects of glyphosate on shrimp
      • THE COASTAL PEOPLE EPIGENOME (ChildrenENCODE) PROJECT >
        • Shrimp Scampi
        • glyphosate health effects >
          • glyphosate and congenital malformations
          • glyphosate and non-alcoholic fatty liver disease
          • glyphosate and non-Hodgkins lymphoma
  • ONE HEALTH NEWS!
  • PUBLICATIONS
  • MEETINGS - ADWARDS
  • CONTACT US